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Pemurnian Enzim Bromelin dari Buah Nenas dengan Kromatografi Penukar Ion Karboksi Metil Selulosa

Nama Pengarang : -
Nama Majalah/Jurnal : Jurnal Penelitian Sains dan Teknologi
Volume / Nomor Edisi : -
Nomor Halaman : 79-85
Abstraksi Narasi : Bromelin is one of the proteolytic enzymes. In order to obtain the high activity and pure enzyme, the enzyme was purificated as follows: isolation of the enzyme from other components, 50% w/v ammonium sulphate precipitation of the enzyme, and carboxy methyl cellulose (CMC) ion exchange column chromatography. The enzyme activity determination was based on the amount of amino acid and simple peptides formed. The protein content was determined by Lowry method. The result showed that specific activity of the CM-cellulose column chromatography purified enzyme was 120 U/mg, degree of enzyme purity 57.4 fold, the recovery of 38.2%, optimum pH at 6.0, optimum temperature at 55 degree, and optimum incubation time at 10 minutes.

Pemupukan Kalium dan Serangan Penggeruk Padi Kuning (Scirpophaga incertulas Wlk.) pada Padi Walik Jerami di Lahan Sawah Tadah Hujan Kahat Kalium

Nama Pengarang : -
Nama Majalah/Jurnal : Jurnal Penelitian Sains dan Teknologi
Volume / Nomor Edisi : -
Nomor Halaman : 241-245
Abstraksi Narasi : Average yield of walik jerami rice in rainfed lowland areas is relatively low, about 1.0-1.5 t/ha. It`s due to various factors, namely low in soil fertility, especially poor in K; yellow stemborer damage during crop generative phase; and drought during grain filling phase. The experiment was conducted in Jakenan Experiment Station of Research Station for Food Crops during 1995`^S dry season. The purpose of this study was to understand the effect of K fertilization on whitehead incidence on reproductive phase of walik jerami rice crop, and to compare yellow stemborer damage on IR 64 and IR 36 varieties. This experiment used split plot design replicated three times. Popular varieties at farmers field (IR 64 and IR 36) was as main plot, and potassium rates (0, 20, and 40 kg K2O/ha) as subplot. Percentage of whitehead affected by yellow stemborer Scirpophaga incertulas IR 64 was relatively higher than that on IR 36. Larvae weight on IR 64 was also higher than that on IR 36. Application of K fertilizer and its interaction with rice variety were significantly different to intensity of stemborer damage and larvae weight. The increase of K fertilizer rate was significantly reduce yellow stemborer damage and larvae weight. This study was regionally specific, because was done in soil with K deficient and rice crop was quite responsive to K fertilization. So, it should be studied continously.

Pemilihan Peubah Berdasarkan Aturan Penghentian

Nama Pengarang : -
Nama Majalah/Jurnal : Jurnal Penelitian Sains dan Teknologi
Volume / Nomor Edisi : -
Nomor Halaman : 113-117
Abstraksi Narasi : The aim of this study is to determine important variables that can preserve the configuration of original data through Principal Component Analysis. Selection variable is based on the stopping rule. Two sets of chemistry data on the degraded mineral soil and peats are used in this study. Based on the measure for preservingthe configuration, R^2 values, this methods have the capability in representing the original data.

Mikrofiora pada Fermentasi Daging Buah Durian (Tempoyak)

Nama Pengarang : -
Nama Majalah/Jurnal : Jurnal Penelitian Sains dan Teknologi
Volume / Nomor Edisi : -
Nomor Halaman : 140-147
Abstraksi Narasi : The results of the rresearch showed that were differences from mold, yeast and lactic acid bacteria population in generation time and specific growth rate during fermentation durian fruit flesh (tempoyak). The generation time of mold yeast and lactic acid bacteria population consecutively were 3.8 h, 1.7 h and 2.1 h. moreover, the specific growth rate (myu) of mold, yeast and lactic acid bacteria population consecutively were 0,18 h^-1; 0.39h^-1; 0.33 h^-1. The lactic acid bacteria was dominated by L. fersantum (B1) and L.casei (B2) The yeast population was deminated by K. marxianus Var. dobzhansSkii (Y1), K. marxinus Var. drosphilarum (Y2), and K. lactis (Y3). The mold population was dominated by Aspergillum and Penicillium.

Konsumsi Oksigen dan Aktivitas Lokomotif Udang Galah di Bawah Medan Elektromagnet

Nama Pengarang : -
Nama Majalah/Jurnal : Jurnal Penelitian Sains dan Teknologi
Volume / Nomor Edisi : -
Nomor Halaman : 226-232
Abstraksi Narasi : The effects of electromagnetic fields on oxygen consumption and locomotor activity of freshwater shrimp (Macrobrachium rosenbergii) had been studied Shrimps of juvenile stage were kept in solenoid of different fields: 0,10^-1, 1, 10^2, 10^3, 10^4, 2x10^4, and 3x10^4 myu Tesla. Oxygen cunsumed during exposure was recorded. Locomotor activity was investigated by recording the speed of movement and the total time spent by shrimps in one-hour period. The results showed that fields of 10^-1, 10, 10, 10^3, and 3x10^4 myu Tesla significantly lowered oxygen consumption. Movement speed was decreased by 10^-1, 10^3, 2x10^4, and 3x10^4 myu Tesla. Total movement time tend to increase under the fields of 10^-1, 1, 10^2, 10^-3, and 2x10^4 myu Tesla, but decrease under 3x10^4 Tesla. It cans be conclude that the electromagnetic field effects were multiphasic, and tend to decrease the oxygen consumption and locomotor activity of freshwater shrimp.

Koefisien Pertautan Fluks-Gaya Sistem Difusif Isotermal Larutan Terner NaNo3-Kno3-H2O

Nama Pengarang : -
Nama Majalah/Jurnal : Jurnal Penelitian Sains dan Teknologi
Volume / Nomor Edisi : -
Nomor Halaman : 55-66
Abstraksi Narasi : The thermodynamic flux-forces relationship in diffusion processes was converted into the Fick`s equation. The flux-forces relationship in diffusion processes designated by the appearance of diffusion coefficients Dik. These coeficients are related to pheno-menological coefficients Lik, that are determined through the experimental data. The experiment based on the method of Linder at al was modified with Crooks`s method and Bird`s. The transformation tools for converting the Dik to Lik were derived from the Debye-Huckel`s theory and Wendt`s method. So the transformation tools for predicting Dik and Lik values have been formulated from Wendt`s method. The experimen were carried out for ternary system of NaNO3-KNO3-H2O at 25 degree, with the molar compositions of NaNO3:KNO3 as 0,0025:0,01, 0,005:0,0075, 0,0075:0,005 and 0,01:0,0025, were to determine the cross-term diffusion coefficients (D12 and D21) and to evaluate the Onsager reciprocity relation (ORR) and phenomeno-logical laws. The main-term diffusion coefficients NaNO3-H2O and KNO3-H2O were determined from each binary system, with initial concentrations of 0,0025M, 0,005M, 0,075M, and 0,01M. The experimental results show that diffusion coeficient values are in agreement with either the values of prediction and that of the literatures. The tendency of diffusion coefficient found in this research are quite similar to those reported by Rard and Miller. The validity of Onsager reciprocity relation (ORR), that is Lik=Lki, is obeyed and verified. The experimental research shows that the tenary system chosen as an experimental model obeys the (L12+L21)^2

Koefisien Determinasi Modifikasi sebagai Alat Ukur Ketepatan Verifikasi Suatu Model Simulasi

Nama Pengarang : -
Nama Majalah/Jurnal : Jurnal Penelitian Sains dan Teknologi
Volume / Nomor Edisi : -
Nomor Halaman : 129-134
Abstraksi Narasi : A coefficient of determination (R^2) is used to determine whether the fit of correlation between dependent and independent variable or the fit between data and prediction/model. He coefficient of determination is valid only if mean data. The mean data is not necesserilly equal to the mean of model, if not the coefficient of determination has to the modified as a valid measure of fit. In this study the coefficient of determination (R^2) was modified to be R^2m, by using a simulation model for black pepper drying process. Data were collected from three layer of black pepper in the drying process; that were bottom, middle, and top layers. It was found that R^2m were 0.970, 0.956, and 0.907 respectively for the bottom, middle, and top layers.

Kinerja Elektroda Enzim Glukosa Oksidase sebagai Sensor pH secara Potensiometri

Nama Pengarang : -
Nama Majalah/Jurnal : Jurnal Penelitian Sains dan Teknologi
Volume / Nomor Edisi : -
Nomor Halaman : 30-36
Abstraksi Narasi : The method for ammobillizing glucose oxidase (GOD) on cellulose acetate (CA) membranes on an electrode surface platinum wire were prepared and studied. The research is aimed to know the character of GOD monitored by potentiometric. The characters were respons time, Nernst factor, linearity, detection of limit, and life time. The research glucose standard solution and potential measured against Ag/AcCl refference electrode. It`s Nernst factor of 59.09 mV/decade concentration, response time 50 to 60 sec. The response is linear as concentration glucose 10^-6M until 10^-1M, correlation coefficient a 0.9998. This electrode can be used up to of 40 days.

Kesesuaian Polen dan Stigma dalam Mengontrol Polinasi pada Semangka [Citrullus lanatus (Thunb.) Matsum and Nakai]

Nama Pengarang : -
Nama Majalah/Jurnal : Jurnal Penelitian Sains dan Teknologi
Volume / Nomor Edisi : -
Nomor Halaman : 282-288
Abstraksi Narasi : Most of studies done in reproduction of watermelon (Citrus lanatus) have been focused on the pollen and stigma which related to each other in tem of pollination process. The activity of the pollen and stigma affects to the formation of pollen tube, in which sperms are ready to fertilize. The readiness of stigma is determined by secretion made to receive pollen during pollination. Stigmatic exudate of C. lanatus is formed oneday before anthesis with the most abundant of exudare during anthesis. Stigma pollination during anthesis stimulates stigmatic secretion. The next exudate is secreted again after 10 minutes from the first pollination, and it is lasted for another 30 minutes. The elevation of such secretion in responding to pollination assured hidration and formation of pollen tube. C. lanatus pollens could form pollen tube once is attached to stigma of C. lanatus during pollination, in term of fruit to mature after fertilization occured. Pollination of C. lanatus stigma by pollen from different species resultes in inhibition of pollen tube formation in that stigma or in a stylus, thus there will be no fertilization, as consequences no fruit is formed.

Keragaman Fitonematoda Parasit pada Pertanaman Kopi (Coffea robusta) secara Monokultur and Polikultur

Nama Pengarang : -
Nama Majalah/Jurnal : Jurnal Penelitian Sains dan Teknologi
Volume / Nomor Edisi : -
Nomor Halaman : 271-281
Abstraksi Narasi : The research of diversity of plant-parasitic nematodes in coffee (cofea robusta) grown as monocultre and multiple cropping were done at coffee plantation area at "Loka Pengkajian Teknologi Pertanian" Natar, in the south of Sumatra. He research was conducted in July until October 1977. Many samples of the soil and roots of coffee plants were collected from coffee grown aas monoculture and coffee grown as multiple cropping (coffee and pipper and vanilie; coffee-peanut-tunggak bean). the result of research indicated many genus of plant parasitic nematodes in the soil and roots of coffee grown as monoculture were more survive than that genus in coffee grown as multiple cropping. The highest of reltive dominacy of the plant parasitic nematode in coffee was pratylenchus.
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